When your friend screws you over.
I loaned Seth some money and he totally spooed me by not paying me back for over a year.
by The coalition for freedom November 16, 2012
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1- The male ejaculatory fluid, especially when deposited somewhere other than the woman's vagina

2- The act of a male ejaculating
The male porn star got spoo all over the woman's face.

Oh, baby, I'm about to spoo!
by Batrastard April 19, 2003
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Spoo is/are (the plural of Spoo is Spoo) little white pasty creatures mentioned in passing in Babylon 5 as being a delicacy of the Centauri. They are mealy little critters which don't do much in the way of anything.
"Ugh, it's terrible. And the Centauri love this stuff?"
"Eat it by the truckload."
"And what do you think of the ham?"
"Tastes like chicken."
by Atalhlla October 27, 2004
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(s-p-oooooooo) (the oooooooo is said in a high pitched voice)
It is the word 'oops' backwards and is used to describe something that is small and cute.
Look at that tiny grape. We will now refer to it as spoo.
by Exabanche December 28, 2018
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A substance that male vampires ejaculate in place of sperm, which is absent from their bodies. Spoo, like all other liquids in a vampire's body (besides the blood that they drink) is venom.

It tastes and smells delicious, and there is a small line of possible spoo-products being discussed. Those products include, but are not limited to:

Shamspoo
KY Spooelly
Hairspooray
Spootrogena Body Lotion
Sparklyspoo Body Lotion
Spoo Flu Treatment
OOOOOOOOOOOH! d'Edward
Have you been on the Vampire Mating thread lately? They're talking about spoo!
by Random Lexiconer December 23, 2006
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The male ejaculatory fluid, also known as "spunk" which is a gooie substance. Hence the amalgamation of the two words "spunk" and "goo" to form the word Spoo.
"I'm going to spoo on you."

"oh no I've got spoo on me!"

or just say the word loudly and proudly "SPOOOOO"
by bastard_pop February 09, 2007
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Oops spelled backwards. This way, you can say oops without saying oops.
spoo, I dropped my milk
by Fragbait November 19, 2003
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