jimmies – feelings / thoughts / ego
rustled – hurt / disturbed
unrustled – peaceful / calm
As per Know Your Meme, the expression originated from 4chan in late 2010, and is used by trolls to "convey feelings of discontent or discomfort in response to someone else’s post in imageboards and discussion forums".
The expression is an alternate form of "u mad bro?".
Victim: What the heck is your problem!?
Troll: Are your jimmies rustled?
Victim: ...What is this I don't even—
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