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1.
When you do something that’s going to end in certain failure but you decide to do it because it’s your obligation to do so. It’s also a very British thing to do.
Can be used in a video game context when you sacrifice yourself for the better of the team.
Variant : suicide mish
Dude1: Cya dude im going on a suicide mission and gna call my ex up about going out again.
Dude2: Instant death.

Dude1: No he’s got a rocket launcher.
Dude2: Don’t worry I'll go outside on a suicide mish and waste his ammo.

Dude1: Dude I got this girl into my room and I was so horny i asked her to suck me off, I thought i was on a suicide mission, but she unzipped my pants and sucked it like a pro whore.
Dude2: Lucky fuck, can I have her number?
by Andy2billion July 03, 2005
 
2.
The Collector Base/ Ending of Mass Effect 2.
Commander Shepard didn't survive the suicide mission.
by LukeMCFC 141 April 01, 2012