Top Definition
a motorcycle gang formed by and for women, primarily lesbians. lesbians from all over the butch-femme scale are encouraged to be and are members. the dykes on bikes website states their mission to be "to support, encourage and motivate women motorcyclists everywhere."
Rachel: Are you going to pride this year?

Heather: Yeah, my mothers gave me a total guilt trip about watching them ride with the Dykes on Bikes.
by nutthb November 20, 2007
A motorcycle gang formed by a group of man-like lesbians.
Ann and Fidelma are in the Dykes on Bikes.
by Da November 07, 2003

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