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do you bite your thumb at us sir 

used in Romeo and Juliet. during that time period it was the equal of flicking someone off= highly offensive.
"do you bite your thumb at us sir?"
"yeah, wanna make something of it?"

I bite my thumb at thou! 

Actually, Shakespeare never used this specific phrase. If he had, the gramtically correct way to say it would be "I bite my thumb at thee!"
I will bite my thumb at them, which is disgrace to them if they bear it.

--Shakespeare (from Romeo and Juliet)

I bite my thumb at you 

The Medieval Middle finger.

Fuck off.
John: Your such a prick Erin.

Erin: I Bite my thumb at you *bites thumb in johns general direction*

John takes offense and stomps away.
I bite my thumb at you by Major. M January 20, 2009

I bite my thumb at thou! 

It pretty much mean fuck you in lamens terms, or a different way to flip someone off back in the day
I bite my thumb at thee for being such a disgrace!
I bite my thumb at thou! by Jaja November 30, 2003

I bite my thumb at thou! 

This, actually means that you want to start a fight. A looong time ago people used to "bite" their thumb at someone to show that person you don't actually care about them.
I bite my thumb at thou!. I completely disagree with you all the way.
I bite my thumb at thou! by luciana September 29, 2005

I bite my thumb at thou! 

It's an old English term for "Fuck you!" Is used in many of Shakespeare's plays.
I bite my thumb at thou! Thou hast beseeched thy family!