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1.
Anti-humor and anti-jokes (also known as unjokes) are a kind of humor based on the surprise factor of absence of an expected joke or of a punch line in a narration which is set up as a joke.

Comedians known for their anti-humor are Norm Macdonald, Ted Chippington, Neil Hamburger, Corey Mystyshyn, and Bill Bailey.
ANTI-JOKE: (anti-humor)

Two atoms walk down the street, Silver and Iron. Suddenly, Iron screams and keels over on the floor, coughing up blood and a strange little ball that vanishes in an instant. Silver asks him what happened.
"I...I think I just lost an Electron."
Silver is taken aback. "Are you sure?"
Iron turns to him slowly. His eyes are pulsing red and veins are showing all along his shiny face. Clouds roll in. Darkness falls, thunder cracks in the distance, the light creating a sharp profile of Iron before he falls back into a darkness pierced only by his gleaming eyes. Finally, he opens his mouth, and slowly he wraps his word around every syllable, savouring it. He says... "Of course I'm fucking sure!"

REAL JOKE:

Two atoms walking down the street.
One says "I've lost an electron."
Other says "Are you sure?"
First says "I'm positive."
by PieInMyEye November 05, 2009
 
2.
Anti-humor is best described as a joke or image that is intended to be funny, but instead inspires anger in its witness or witnesses.

Something that is so unfunny that it pisses you off.

Talking animal jokes, America's Funniest Home Videos, the majority of humorous chain emails, and Cuba Gooding Jr. comedies are all examples of anti-humor.
I actually tried to sit down and watch an episode of Will and Grace, but I could only get through twelve seconds before the relentless attack of anti-humor pissed me off so much that I smashed my fucking television.
by Scott Wood - Film Critic February 26, 2007