The video gained even more popularity from a RWJ(raywilliamjohnson) video.
2. The best comeback for anything.
You: YOU AIN'T GOT NO PANCAKE MIX!
2. Your common jackass: Your a weak minded little fag with no life.
You: YOU AIN'T GOT NO PANCAKE MIX!!
The reason for him deciding to say that is unknown. One can assume that he simply chose the phrase at random. Whatever the reason for him saying it, he managed to upset the lady. Lulz ensued.
Kid: You ain't got no pancake mix!
Fundie lady: In the Bib-....
Kid: THERE AIN'T NO PANCAKE MIX IN THERE!!!!1
Fundie Lady: Stop shouti-...
Kid: YOU'RE DECEIVING THESE PEOPLE!!1 THERE AIN'T NO PANCAKE MIX IN THERE!!1
Random civilian: You Ain't Got No Pancake Mix!
Preacher: What the heeeee-ated place down under
"Why do I sit around Indian-style on my hardwood floor, yelling out to myself, 'You ain't got no pancake mix!'?"
It was coined by RayWilliamJohnson on YouTube.
Person 2: "YOU AIN'T GOT NO PANCAKE MIX!"
Person 1: "..."
2: A way to let somebody know that they ain't got no pancake mix left, and should probably go to the store.
You: You ain't got no pancake mix!