Absurd, stream-of-consciousness phrase used by Zach Galifianakis in a stand-up routine in Luna Park, West Hollywood, California in January 1999. The term has come to be a broader vehicle for closing an argument or putting a speaker off balance. This use can be viewed on YouTube by searching for "Zach Galifanakis Luna Park 1999". The line occurs about about 5:31 into the routine.
From Zach Glifianakis' 1999 Luna Park, West Hollywood comedy routine:

"Why do I sit around Indian-style on my hardwood floor, yelling out to myself, 'You ain't got no pancake mix!'?"
by MrFrankBlack November 2, 2011
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a phrase usually used to annoy and stop preachers from spreading the word of god by saying everyone goes to hell. most often associated with the youtuber "Piscator11" when he first exclamed that a woman aint got no pancake mix
Preacher: You all going to hell!

Random civilian: You Ain't Got No Pancake Mix!

Preacher: What the heeeee-ated place down under
by YAGNPM June 26, 2010
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1. The perfect way to shut up a annoying preacher person who shows up out of nowhere. Proven from a popular youtube video titled, 'You ain't got no pancake mix.'.

The video gained even more popularity from a RWJ(raywilliamjohnson) video.

2. The best comeback for anything.
1. Annoying person: If you do not believe in God you will go to hell, and burn for ever.

You: YOU AIN'T GOT NO PANCAKE MIX!

Annoying person:........

2. Your common jackass: Your a weak minded little fag with no life.

You: YOU AIN'T GOT NO PANCAKE MIX!!
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One day, a Christian extremist fundie lady made the mistake of preaching to a group of students, and threatening them with hell. One of the students had enough of her non-sense and decided to do something quite strange and unpredictable. He approached her and shouted "You ain't got no pancake mix!" and other variations of the phrase repeatedly. Every time she opened her mouth, he would interrupt her, similar to Kanye West.

The reason for him deciding to say that is unknown. One can assume that he simply chose the phrase at random. Whatever the reason for him saying it, he managed to upset the lady. Lulz ensued.
Fundie lady: Repent or you'll go to hell. Jesus cries when you fap. Blahblahblahblah...

Kid: You ain't got no pancake mix!

Fundie lady: In the Bib-....

Kid: THERE AIN'T NO PANCAKE MIX IN THERE!!!!1

Fundie Lady: Stop shouti-...

Kid: YOU'RE DECEIVING THESE PEOPLE!!1 THERE AIN'T NO PANCAKE MIX IN THERE!!1

etc. etc...
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1: A way to get somebody your annoyed at to stop talking.
2: A way to let somebody know that they ain't got no pancake mix left, and should probably go to the store.
Protester: Abortion is muder!
You: You ain't got no pancake mix!
Protester: .....?
by wehttam16 February 2, 2011
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Meant to be used to throw someone off. If there's a serious argument, say that, and you'll win just by pure awesomeness. Occasionally the word "Bitch" can be thrown at the end of it.

It was coined by RayWilliamJohnson on YouTube.
Person 1: "Personally, I believe that god is going to smite every last one of the sinners--"
Person 2: "YOU AIN'T GOT NO PANCAKE MIX!"
Person 1: "..."
by epic-win-fail June 25, 2010
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A phrase used to show dissatisfaction or discontent with someone or something. It was made famous by the comedian Zach Galifianakis and its commonly seen in YouTube video posts.
Did you get the food for the party?" "No, why should I be the one to do it?" "You didn't?! You ain't got no pancake mix!
by studmuffin4567 June 25, 2010
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