1. Female webmaster.
2. A female who programs, designs, and/or creates a web site.
The sorority's web site is not working properly; I will contact the webmistress of the problem.
by Cryptic March 05, 2004
A wife who loses her husband's attention and love to the more seductive Internet.
After Jack began spending more time on status updates and tweets, his wife realized she'd become a webmistress.
by Mohit Hira February 08, 2010

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