The granddaughter of the 1st Hokage of Konoha (founder of the hidden village itself), she is well-respected and most likely belongs to a noble clan. She lost her younger brother and lover during the war and that subsequently causes her to develop hemophobia. Tsunade manages to overcome the fear during her confrontation with Orochimaru, right before she accepts the position of Hokage.
She owns a priceless crystal necklace, an heirloom from the 1st Hokage. It is believed that the bearer of the necklace, other than Tsunade, will die. However, Naruto won the necklace from Tsunade in a bet and is able to break the curse surrounding the item.
Tsunade is addicted to gambling, and is also known as the Legendary Sucker due to her extreme bad luck in the arena.
As a medical nin specialist, she is able to cast special jutsus that keeps her looking young and beautiful. Her skills in the medical field is also the reason why Orochimaru tries to enlist her help in reviving his arms that the 3rd Hokage, also known as the Sandaime, has sealed using the Shikifuujin rendering it unable to perform jutsus.
Her apprentice, Shizune and pet pig, Ton Ton, accompany her during her travels to gambling hotspots.
After becoming the Godaime, Tsunade accepts Haruno Sakura as her next apprentice.
Tsunade has a soft-spot for Uzumaki Naruto, and sees the dream of both her younger brother and her lover living in him. Their outward relationship, however, consists of shouting matches, insults and physical fights.
Tsunade enjoys gambling a little too much.