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1.
Ejaculating into the end of someone else's penis.
Last night, I caught Gary threading the needle of some homeless guy in the park.
by Gary Needle April 20, 2013
 
2.
The act of killing someone in a shooter game by shooting the bullet through a narrow space at distance . Usually done with a sniper rifle and with only one shot.
Gamer : Dude i was playing Bad Company 2 and all i could see was this campers scalp about half the map away between two hummers through my scope one shot one kill.

Gamers Friend : Dude that's called threading the needle your a bad ass!!
by Cadmb3 March 31, 2011
 
3.
While driving,walking,or running you weave in and out of obstacles (other cars, people, etc.) in your path.
Last night while driving I was threading the needle an made it home in half the time!
by Eliot The Great July 15, 2014
 
4.
Trying to have sex while flaccid
"I was so drunk last night I was practically threading the needle with that girl."
by Cohen95 July 10, 2008
 
5.
A person is taking a dump, and a person has to piss. So the person pisses between the poopers legs without splashing or dribbling.
Last night I was so drunk I was threading the needle.
by Jeff Bohagen March 18, 2009