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1.
Quote from The Matrix, stating that the spoon Neo holds in his hand is not real, along with everything around him. Because of this, he learns how to manipulate it with his mind.

When people use it on the internet, they usually use it as a means of non-sense.
"There is no spoon..." ~ Neo

"OMG THERE IS NO SPEWN" ~ Thousands of dweebs on the internet
by 8-Bitty April 17, 2004
 
2.
A phrase popularized by Keanu Reeve's character Neo in The Matrix, acting as a turning point for Neo's mentality regarding the reality of the Matrix.

'There is no spoon' implies that, despite all visual, physical, or mental evidence to the contrary, the thing in question does not exist.
A man walking in the desert sees an oasis appear as from nowhere. "There is no spoon." And he walks away.

*

"Dude, I just saw Bigfoot! Man, he was right there!"

"There is no spoon."
by LouisTsunami November 12, 2009
 
3.
A quote from The Matrix, spoken by Neo in reference to a bit of wisom he learned from a child bending spoons with his mind. It signifies that a person cannot truly manipulate reality, a person can only manipulate him/herself. Only when a person changes him/herself can he/she change reality.
Do not try and bend the spoon. That's impossible. Instead, only realize the truth... THERE IS NO SPOON. Then you will see that it not the spoon that bends, it is yourself.
by Geek3 June 22, 2011
 
4.
A line made popular by the movie The Matrix, in responce to someone asking for, holding, or even talking about a spoon.
"Dude, can you hand me a spoon?"
"There is NO SPOON!"
by MatrixFreak January 20, 2004