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5.
It's an acronym for 'total waste of time', often used on the internet, to describe things (pictures, videos, etc.) that someone wished they hadn't watched.

Refer to: RickRoll.
Well, that was a TWOT.

(Response to a message): TWOT!
by Dutty4u August 04, 2008
93 110
 
1.
a female pussy
clean that twot w/ a douche
by antony November 14, 2003
387 289
 
2.
'The word on the street'
Meaning a rumor or something known in the grapevine. Mostly used in the areas around Bledmeer and Benowyc.
or 'The way of the Stams'
A way to refer to gangs who pick on smaller gangs or individuals.
Sometimes used as an affirmative exclamation.

"Hey Jimmy, twots is that you're into cats"
Or
"Who said I hung around bridges all day?"
"Twots"
by Onelith June 20, 2006
9 7
 
3.
a female who has tattoos and also have a nice pair of titties
Me and a friend was at a bar drinking our beer when this fine looking Two T's came in. She was a brunette who had tattoos on her upper arms and her breasts was popping out of her tank-top.
by Thieng April 29, 2011
6 7
 
4.
Someone who tweets a lot.
"Yeah, she's funny, but she's a super twot."
by falstar July 22, 2012
19 32
 
6.
Someone, who without effort, makes a right proper useless waste of space of himself. Not annoying, just a useless twot.
When he died it was a relief to us all... the useless twot.
by clemstar February 27, 2009
55 75
 
7.
Singular past tense verb representing a message on the micro-blogging service, Twitter.
At 140 characters, that was the biggest twot I've ever seen!
by CaptainAnonymous February 05, 2009
118 141