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1.
It's an expression use to describe the harsh feelings of wanting to use the toilet really badly.

Is a game where two or more people try to hold out from craping in there pants the longest. The winner is the person who does not shit themselves.
You know how a turtle sticks his head in and out of its shell? Well that's what I'm doing to my ass, I'm playing turtle with my crap.
by B0$$MAN January 08, 2010
 
2.
This is what happens to a man's penis and testicles when he gets scared, nervous or cold. His junk has major shrinkage.
I took my pants down to show her my big unit, I guess I was nervous and it was playing turtle on me.
by You Bet May 09, 2009