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1.
On youtube, a video where you see a red engineer on dustbowl running up to the camera, then say "Nope".
His hat then floats up in the air, then the neck extends to fit the head back into the hat.

Oftenly used as a funny no.
1: Spy is not our spy!
2: Nope.avi
by Metalic Wolf May 20, 2011
 
2.
1. On youtube, a video where you see a red engineer on dustbowl running up to the camera, then say "Nope".

His hat then floats up in the air, then the neck extends to fit the head back into the hat.

Oftenly used as a funny no.

2. Occasionally used to describe a deep fear stemming from the current situation, (whether it be a video, movie, or game.)
the "Nope" also indicates a denial of the current situation that has put them in such fear...
1.
"That Engineer is a spy!"
"Nope"

2.
"and then a skeleton popped out"
"NOPE.avi"
by SgtPerkins September 09, 2011