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3.
Little Sisters are genetically enhanced female children between the ages of about 4 and 7 who roam the halls of Rapture looking for ADAM. When they come across a body containing ADAM (an "Angel") they stop and using their syringe needles they extract the ADAM from the body. They then move on to the next body, sipping the blood/ADAM mix in their syringe needles. Once the blood/ADAM mix enters their bodies the ADAM is separated by the ADAM slugs living like parasites inside of them.
Now should you get your hands on a Little Sister (that is if you can get past their protectors the Big Daddies whom they refer to as Mr. Bubbles or Mr. B), you have a few options. You can save the little sister by killing the slug in her. This will turn her into a normal little girl, you will get whatever ADAM she has on her and she will be forever in your debt which can come to an advantage later. The second option is you can harvest her which will result in a maximum ADAM gain. This can lead to more plasmids and will make it easier for you to survive. She will not survive the process however.

Your final option can only be achieved if you are also a Big Daddy. You can adopt her and act as her protector. Bring her to the "Angels" and keep her safe while she gathers ADAM. She will then give you a portion of her ADAM and after two gathers you can choose to save or harvest.
Jack approached the crying Little Sister slowly, Tenenbaum's and Atlas' voices echoing in his head.

Hurry Mr. B! Angels don't wait for slowpokes.

I can see angels!

Get him Mr. B!
by Grace Holloway March 01, 2010
 
1.
A woman who is officially affiliated with a fraternity. Little Sisters are usually given the fraternity's letters to wear after a sort of initiation, but they don't undergo a Pledge period and have limited rights and privileges within the frat. They are not considered to have equal standing with the Brothers and don't participate in rituals or attend meetings.Usually long time friends of the guys in the frat and/or the serious girlfriend of a Brother, a Little Sisters provide support to the fraternity. They assist in Rush events, bake cookies, and generally make the guys look good.
After two years of baking brownies and cleaning up after the guys Kari was made a Little Sister of the fraternity.
by Oh Donna May 21, 2008
 
2.
The most annoying beings on the face of the planet. They strive to drive you absolutely nuts. And when you get mad at them for driving you absolutly nuts, they have tantraums.
-Big sister: ANNA!!! WHAT DID YOU DO WITH MY BRA???
-Little sister: Well I had to use SOMETHING to hang my teddy bear with!
-Big sister:AAAAAAAAAAAAAA AAAAAAAAAAAAAAA AAAAAAAAAAAAAAA AAAAAAAAAAAAAAA AAAAAAARRRRR RRRRRRRRRRGH!!! *hits little sister*
-Little sister: *bursts into tears* NORA HIT ME FOR NO REASON!!!
by NoNo Claire December 17, 2006
 
4.
Girls who hang around a fraternity in hopes of finding a boyfriend/husband. Usually they find neither but end up geting fucked by numeroius members of the fraternity.
I hope this semester we get some new little sisters!
by yo mama September 16, 2003
 
5.
the victim of an over protective male influence known as a "big daddy"
Zaxq: poor shelbie has turned into a little sister!
Drew: I Know kyle's being such a big daddy!
by xXPyramidheadXx3 February 17, 2010
 
6.
the most annoying little losers in the world who look up to you and wont stop leaving you the fuck alone!
little sister follows you tell her to fuck off, wont stop tell parents older sibling gets blame.
by J'aDoRe JUiiCY September 25, 2006
 
7.
annoying little person who interupts a couple while they are trying to fuck without getting caught by the parentals in the next room
His damn little sister keeps taking away my "me time".
by S.S.13A303 February 25, 2005