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1.
Similar to "Blue Balls," this is the phenomenon that occurs when a fan of the show LOST is anticipating the next episode or the outcome of the most recent pre-commercial cliffhanger, only to be let down when they discover that their questions are left unanswered.
Brian: "I thought for sure that they were finally going to reveal the Man In Black's real name."
Melinda: "Sounds like you got a case of LOST Balls!"
by brifrye June 02, 2010
 
2.
When you are about to do something and suddenly lose your nerve to complete the task at hand.
I was about to jump over my moms house on my bike but I lost balls at the last second when I realized that I don't have health insurance.
by Dirty D-ness November 17, 2007