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1.
The Gus Bus is Gustavo Sorola, the voice of Simmons of Red vs Blue, but the term Gus Bus came from a comic strip from Penny Arcade. (PAX)
"The Gus Bus never runs out of gas"

"Who will wrestle Gus?"
by Caboose343 October 16, 2005
 
2.
A fictitious "bus" named after Auburn's current head football coach, Gus Malzahn, which that could mean any of the following:

1. The Auburn football team
2. The vehicle carrying the Auburn football team
3. A vehicle carrying a bunch of Auburn football fans
4. A general mood of optimism among Auburn fans
You'd best get on the Gus Bus or you'll get left behind!
by au2013tiger December 30, 2013
 
3.
The act of going to Wembley Football Stadium on the 'Gus Bus,' specifically used by Sunderland AFC fans when they reached the Final of the Capital One Cup in 2014 as their manager was Gus Poyet at the time
by SAFC fan January 25, 2014