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1.
1) An observation: said to someone who is getting all up in your face over some trivial matter.

2) An exclamation: used to indicate someone is offending your sensibilities, or mightily trying your patience.

3) A warning: said to indicate that someone is pushing an issue to uncomfortable limits to which you do not want to "go", and to invite them to know their place.

Particularily amusing when said to men with short or no hair, for the incongruity of it all.

Often suffixed with "bitch", to top it off nicely.
"You've been on my ass all day about this. Time to fix yo' weave, bitch!"
by SeaBat June 09, 2006